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TETRASTRIPS (Rota, Corona, E. coli F5, Cryptosporidium ) BIO K 156 ( 5 combined tests)

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Diarrhoea is a major cause of mortality in young cattle under one month.
Bovine neonatal gastroenteritis is a multifactorial disease.
It can be caused by viruses (coronavirus or rotavirus), by bacteria: (''Salmonella'', pathogenic strains of ''E. coli'') or by protozoa such as ''Cryptosporidium parvum''.

Coronavirus and rotavirus are often associated with episodes of neonatal diarrhoea.
''Cryptosporidium parvum'' is also frequently isolated in faeces, where it can be present in very high quantities.
It can persist for a long period in the environment.
F5-positive enterotoxigenic E. coli is frequently isolated in under-three-day-old calves, particularly in colostrum-deprived calves or in calves fed colostrum that is free of anti- E. coli F5 + specific antibody.

The diagnosis of the etiological agent of diarrhoea can be performed only in the laboratory because the clinical signs do not suffice to distinguish between these different microorganisms.

It is possible to identify these agents by means of different techniques, including culture, staining, electronmicroscopy and floating techniques.
However, these techniques are labour intensive, impractical and time consuming.
These classical techniques have rapidly been replaced by the ELISA technology because of its simplicity and limited laboratory equipment requirements.

The sensitivity and specificity of the ELISA technique for detecting these pathogens is at least as good as that of the more classic techniques, and the results are very similar.

The ELISA technique is rapid and reliable and is particularly suited to the analysis of large numbers of samples.
Nevertheless, ELISA can be time consuming and expensive especially when small number of analysis has to be performed.

Chromatographic vertical flow immunoassay is becoming the gold standard for gastroenteritis diagnosis because of its simplicity, rapidity, sensitivity and specificity.
Laboratory equipment required is limited. Results compared with classical techniques are rather similar in terms of diagnosis and strips are far easier to use.

==Use of the kit== The kit is designed to detect Rotavirus, Coronavirus, ''E. coli'' F5 (K99), ''Cryptosporidium parvum'' in calf stool.

==Reliable Results== The use of monoclonal antibodies as conjugates and to capture the pathogens on the strip ensures excellent specificity and very reliable results.

==Ease-of-Use== *Minimal hands-on-time *Room temperature incubation *Results available in 10 minutes
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This product was added to our catalog on Wednesday 23 December, 2009.