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Test strips for the detection of E. Coli F5 (K99) BIO K 154 (1 X 10 strips)

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Diarrhoea is a major cause of mortality in young cattle under six weeks.
Bovine neonatal gastroenteritis is a multifactorial disease.

It can be caused by viruses : coronavirus or rotavirus, by bacteria : ''Salmonella'' or by protozoan microorganisms such as ''Cryptosporidium parvum''.
In calves younger than three days, F5 (K99) positive enterotoxigenic E.Coli are frequently isolated, particularly in colostrum-deprived calves or in calves which received colustrum free of anti - F5 specifi c antibodies.

The diagnosis of the etiological agent of diarrhoea can only be performed in the laboratory because clinical signs do not allow to differentiate between the different microorganisms.

The E. coli bacteria can be isolated from faeces on an appropriate growth medium.
The Minca medium is often used because allows optimal expression of the attachment factor F5. However, the culture may be unsuccessful if the calf has previously undergone antibiotherapy.
In this case, the strip test or even the ELISA test can be very useful because it will detect the attachment factor F5 even on dead bacteria.
The identification of E. coli bacteria on a growth medium is not sufficient on its own. It must be coupled to a detection test for the attachment factor F5 or the toxin.

The strip test can be used to detect the attachment factor F5 produced by the E. coli bacteria in culture.
The ELISA method is particularly useful when a large number of samples must be analysed. When a small number of analysis has to be performed, chromatographic vertical flow immunoassay is preferable because of its simplicity, rapidity, sensitivity and specificity.
Results compared with ELISA are rather similar in terms of diagnosis and strips are far easier to use.

==Use of the kit== The kit is designed to detect ''E. coli'' F5 attachment factor in calf stool

==Reliable Results== The use of monoclonal antibody as conjugate and to capture the virus on the strip ensures excellent specificity and very reliable results.

==Ease-of-Use== *Minimal hands-on-time *Room temperature incubation *Results available in 10 minutes
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This product was added to our catalog on Wednesday 23 December, 2009.